Isolation and Characterization of Egg Lecithin.

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The Unsaturated Fatty Acids of Egg Lecithin

The work on liver lecithins by Levene and Ingvaldsen’ and by Levene and Simms,z has shown the existence of lecithins which are distinguished from one another by the nature of their unsaturated fatty acids. There were isolated from liver lecithin, two unsaturated fatty acids, oleic and arachidonic, as well as two saturated acids, palmitic and stearic. Hence on the basis of the present findings i...

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Osmotic shrinkage of giant egg-lecithin vesicles.

Osmotic shrinkage of giant egg-lecithin vesicles was observed by phase-contrast microscopy. The vesicles remained or became spherical when shrinking. Small and thick-walled vesicles formed visible fingers attached to the sphere. The water permeability of the single bilayer was found to be 41 micrometers/s. A variety of observations indicate that osmosis induces a parallel lipid flow between the...

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The preparative isolation of lecithin.

Lecithin can be prepared on a relatively large scale, free of colored impurities, by a simple two-column procedure. Commercial crude egg lecithin is partially purified by a single-step passage through an alumina column. It is then purified by a two-step passage through a prepacked, commercial silica gel column. The lecithin is prepared in solvent-free form for weighing by lyophilization from cy...

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Isolation and characterization of sea urchin egg cortical granules

A method has been developed to isolate cortical granules (CG) free in suspension. It involves the mechanical disruption of the CG from CG lawns (CGL; Dev. Biol. 43:62-74, 1975) and concentration of the CG by low speed centrifugation. The isolated CG are intact and are a relatively pure population as judged by electron microscopy. Granule integrity is confirmed by the fact that isolated intact C...

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ژورنال

عنوان ژورنال: Acta Chemica Scandinavica

سال: 1973

ISSN: 0904-213X

DOI: 10.3891/acta.chem.scand.27-3545